Part:BBa_K812051:Design

Golden brick version of pSB1K3 plasmid for Golden Gate and Golden Brick cloning

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2190
  Illegal XbaI site found at 2212
  Illegal SpeI site found at 1
  Illegal PstI site found at 23
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2190
  Illegal SpeI site found at 1
  Illegal PstI site found at 23
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2190
  Illegal XhoI site found at 1040
  Illegal XhoI site found at 2066
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2190
  Illegal XbaI site found at 2212
  Illegal SpeI site found at 1
  Illegal PstI site found at 23
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2190
  Illegal XbaI site found at 2212
  Illegal SpeI site found at 1
  Illegal PstI site found at 23
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BsaI site found at 7
  Illegal BsaI.rc site found at 2206
  

Design Notes

The negative control J4450 has been amplified with new goldenbrick extensions using PCR and re-inserted in pSB1C3 using EcoRI and PstI cloning.

Source

This part is a derivation of the pSB1K3 plasmid from the registry.